Method of producing acetamide derivatives in form of isomer mixture or individual isomers

专利摘要:
The invention relates to a process for the preparation of acetamide derivatives, in particular compounds of the formula I RaR. R -CfiH, - X-NRj-C (O) CH C -3- C (RJ (R, Tc (6) NRo where R, - H, CH, CjHj; R - F, R. 1 5 - H, CHj, CHjO, CH.S, F, Cl, NO, which possess anticonvulsant activity. For the detection of physiological activity, new compounds of formula I were obtained from the corresponding acid halides of substituted 4-oxo-thiazolidin-2-ylidene acetic acid and substituted aniline The process is carried out in an aprotic bipolar solvent at a temperature ranging from room temperature to the boiling point of the reaction mass. Compounds of formula I are isolated in the form of a racemate or individual isomers. The effects of convulsions caused by electric current, pentetrazole and semicarbazide with the administration of compounds of the formula I are 10-100, 40-100 and 50-100, respectively. Toxicity LD L 500 mg / kg of live weight. cm
公开号:SU1251803A3
申请号:SU843735694
申请日:1984-05-07
公开日:1986-08-15
发明作者:Затцингер Герхард；Геррманн Манфред；Фритши Эдгар；Гартенштейн Иоганнес；Бартошик Герд
申请人:Гедеке Аг (Фирма)；
IPC主号:

专利说明:

The invention relates to a process for the preparation of novel chemical biologically active compounds, specifically to a process for the preparation of acetamide derivatives in the form of a mixture of isomers or individual isomers, which have nrotoconvulsive activity.
The aim of the invention is to obtain new acetamide derivatives having a new type for this range of compounds of biological activity — anticonvulsant.
The following examples illustrate the invention
Example 1. (Z) - (3-methyl-4-oxo-thiazolidin-2-ylidene) -N- (2,6-dimethylphenyl) acetamide.
.0.1 mol of (Z) - (3-methyl-A-oxo-thiazolidin-2-ylidene) -acetic acid is dissolved in a mixture of 500 ml of dichloromethane and 5 ml of dimethylformamide and for 45 minutes at room temperature 10 ml of thionyl chloride dissolved in 30 ml of methylene dichloride are added dropwise. The reaction mixture is stirred for another 15 minutes at this temperature and the brown solution is then evaporated in vacuo. The residue is dissolved in 500 ml of dichloromethane. A solution of 0.2 mol of 2,6-dimethylaniline in 30 ml of dichloromethane is added dropwise to the solution obtained after filtration over a period of 30 minutes at room temperature and continued to stir for 4 hours at.
The precipitate formed is filtered off with suction and recrystallized from 50% aqueous acetic acid, washed with neutral water and dried in vacuo.
(Z) - (3-methyl-4-oxo-thiazolididin-2-ylidene) -H1- (2, 6-dimethylphenyl-acetamide, c. 250.4 ° C (decomposition), yield 55% o
The (Z) - (3-methyl-4-oxo-thiazolidin-2-ylidene) acetic acid used as the starting material was prepared as follows.
20 g of (Z) - (3-Me-type-4-oxo-thiazolidin-2-ylidene) -acetic acid t-butyl ester are dissolved in 45 ml of glacial acetic acid and 8 ml of a 33% solution is added. hydrogen bromide in glacial acetic acid
8. (Z) - (3-methyl-4-oxo-thiazolidin-2-ylidene) -N-methyl-N- (2,6-dimetyptocate. After 15 minutes, -55 phenyl) acetamide was added, Т „ш1 . 153-154 with ice water and the liberated acid- (diisopropyl ether), yield 39%. that sucked off. It is rapid decomposition of 9. (Z) - (3-ethyl-4-oxo-thiazolidine) of the target product is carried out with pe-2-ylidene) (2,6-dimethylphenyl) acetocrystallization from isopropanol. The resulting free acid is suitable for immediate further use. In a similar manner, (Z) - (4-oco-thiazolidin-2-yldene) -N- (2,6-dimethylphenyl) acetamide (compound 1) is obtained. Tpl "(decomposition), (50% aqueous acetic acid), yield 58%, Example 2. To 0.020 mol of (Z) - (4-oxo-thiazolidin-2-ylidene) obtained in the same manner as in Example 1) -N - (2,6-dimethylphenyl) -acetamide in 22 ml of 1N sodium hydroxide solution is added dropwise at room temperature.
2.3 g (0.024 mol) of dimethyl sulfate and stirred for 1 h. Then the methylation product is extracted with dichloromethane and, after ordinary processing and crystallization from methanol
receive (Z) -3-methyl-4-oko-thiazolidine-2-ylidene)) - N- (2,6-dimethyl phenyl) - -acetamide (compound 1) with m.p. 250, (decomposition) yield Y8%. According to examples 1 and 2 receive
the following compounds, and the reaction is carried out at a temperature from room temperature to the boiling point of the reaction mixture.
2, (Z) - (3-Methyl-4-oxo-thiazolidin-2-ylidene) -N- (2-chloro-phenyl) acetamide,
Melting point 193-194 ° C (decomposition), (methanol), yield 48%,
3, (+) (Z) - (3-methyl-3-oxo-thiazolidin-2-ylidene) -N- (1-phenylethyl) acetamide, m.p. 122-125 C (propanol-2 / petroleum ether), yield 50%.
4, () (Z) - (3-methyl-4-oxo-thiazolidin-2-ylidene) -N- (1-phenylethyl) acetamide, m.p. 55-60 ° C (dichloromethane),
yield: 45%.
5o (+) - (Z) - (3-methyl-4-oxo-thiazolidin-2-ylidene) -N- (1-phenylethyl) acetamide, m.p. 65-70 s, (dichloromethane), yield 80%.
6. (Z) - (3-methyl-4-oxo-thiazolidine-2-ylidene) -N- (2-chloro-6-methylphenyl) acetamide; M.p. 244-245 C (ethanol), yield 60%.
7, (Z) - (3-methyl-4-oxo-thiazolidin-2-ylidene) -N- (2,6-dichlorophenyl) acet-
I amide, ToPL. 224-226 s (toluene), yield 43%.
8. (Z) - (3-methyl-4-oxo-thiazolidin-2-ylidene) -N-methyl-N- (2,6-dimetip
phenyl) acetamide, T „sh1. 153-154 with (diisopropyl ether), yield 39%. 9. (Z) - (3-ethyl-4-oxo-thiazolidin-2-ylidene) (2,6-dimethylphenyl) acetyl
amide, so pl. 282, (decomposition), (propanol-2), yield A6%.
10. (Z) -N- (2-methoxy-6-methylphenyl - (3-metip-4-oxo-ti-13olidin-2-yl-den) acetamide, mp 158-160 ° C (iso- propanol), yield 40%.
11. (g) -K- (2-methylthio-pepyl) - (3- -methyl-4-oxothiazolidin-2-ylidene) acetamide, m.p. 177-178 s (toluene), yield 48% „
12. (Z) -N- (2-methyl-6-sh tpo-phenyl - (3-methyl-4-oxo-thiazolidine-2-nlidene acetamide, T. mp. 218-219 C (toluene / acetone), yield 44%.
13. (2) - (3-metsh1-4-oxo-thiazolidin-2-ylidene) -a (ietamide. 212215 with (ethanol), 54% yield.,
14. (Z) - (3,5-dimethyl-4-oxo-thiazo-lidin-2-ylidene) -N- (2,6-xylyl) -acetamide, mp: 220-222 ° C (ethanol), you - 58% move.
15. (Z) -N- (2-chloro-6-methyl-phenyl) - - (4-oxo-thiazolidin-2-ylidey) acetamide, m.p. 192-193 with (ethanol), yield 46%.
16. (Z) -N- (2,6-Dichlorophenyl) - (4-oxo-thiazolidin-2-ylidene) -acetamide, m.p. 19 -192 s (ethanol), vkod 42%.
Pharmacological experiments.
In all experiments, the compounds under study were administered in a medium of 0.8% mucus in methylcellulose. The duration of the metered medication was 30 minutes, unless otherwise indicated. The application was performed intragastrically with doses of 20 mg / kg body weight.
Experimental animals (male mice with a body weight of 18-24 g were kept in conditioned conditions. 24 hours before the start of the experiment (if nothing else was indicated) the feed was stopped, but the animals were kept drinking water until the start of the experiment. Each dose was administered 10 animals.
As a comparative substance, the well-known anticonvulsant phenytoin-5,5-diphenyl-2,4-imidazolidinedione was used.
j Spasm caused by isonicotnic hydrazide (1 acid.
The hydrazide of isoic acid of isoic acid (isoniaeid) in a dose of 250 mg / kg subcutaneously was used as a convulsant medication. The observation time was 90 in.
Cramp caused by strychnine.
Sreds-1in nitric was used as a pulverizing seizure.
034
cryst. at a dose of 1.1 mg / kg sc. The observation time was 20 minutes after application.
Electric shock To create an electric shock, an HSE apparatus (type 207) was used to generate electrical pulses. To transfer an electrical pulse to the ears of the mouse, the electrodes were clamped in the form of clips. The duration of the shock was 0.3 s, and the current was 35 mA (self-regulating voltage). The observation time was 5 minutes. Caused by pentetrazole seizure. 140 mg / kg of pentetrazole was given subcutaneously as a means of convulsing. The observation time was 20 minutes.
Convulsions caused by picrotoxin.
Picrotoxin was given at a dose of 15 mg / kg subcutaneously. The observation time was 20 minutes.
Bicuculline causes cramp. Bicuculline purim was used subcutaneously at a dose of 3.0 mg / kg as a convulsant. The observation time was 20 minutes.
Spasm caused by semicarbazid.
Semicarbazide hydrochloride in a dose of 1000 mg / kg subcutaneously was used as a convulsant. The observation time was 90 minutes after application.
The results of the experiments are summarized in table. 1-3.
The data given in table. 1-3 suggest that the compounds according to the invention exhibit a better activity than the well-known anticonvulsant phenytoin.
Acute toxicity (LD.,).
The acute toxicity of the known and new compounds was determined on female mice weighing 18–23 g. All animals were deprived of food 20 hours before the start of the experiment. Drinking water was given in the desired quantity. Each group included 4 animals. The sequence of doses was determined oarphmically. The compounds under study were administered intragastrically in the form of a suspension in 0.88% methylcellulose (20 mg / kg body weight). The observation time for animals was 7 days. At the same time, it was established that the -PD of a known compound is
490 mg / kg, while the proposed compounds have LD ISOO mg / kg.

权利要求:
Claims (1)
[1]
Invention Formula
The method of obtaining derivatives acetam General formula
1 syaz
61 L /; & R4 o - cH-co-isi-xRi. R5
where R, is a hydrogen atom, methyl or
ethyl;
R is a hydrogen atom or methyl; R, R, and R. are the same or different, and each of them is a hydrogen atom, methyl, methoxyl, methylthio, fluorine or chlorine atom, nitro group;
Rg and H, is a hydrogen atom or methyl; X - simple chemical bond
or ethylidene,
in the form of a mixture of isomers or individual isomers, characterized by
Table 1
Comparative studies of anticonvulsant effects on models of 1-7 ED (mg / kg intragastrically, mouse SU
14.0611.3014.88
1,592,345,09
3,411,525,68
8,455,676.85
what
compound of general form - J7
ten
about LSN-SOU
RI
where RI, R and R have the indicated meanings;
Y is a reactive group,
like halogen
, 5 is reacted with a compound of the general formula
at
HN-xX g RS
where R ,, H, R ,, RgHX have the indicated meanings, in an aprotic environment,
bipolar solvent at a temperature from room temperature to the boiling point of the reaction mixture, followed, if necessary, by N-methylation and isolation of the desired
product as a mixture of isomers or individual isomers.
Table Comparative Studies of Anticonvulsant Action

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引用文献:

---

公开号 | 申请日 | 公开日 | 申请人 | 专利标题

---

---

FR2726M|Warner Lambert Pharmaceutical|Substituted thiazolidinone amides.|

---

DE1150985B|1961-03-06|1963-07-04|Warner Lambert Pharmaceutical|Process for the preparation of thiazolidin-4-one derivatives|

---

DE2423725C2|1974-05-16|1982-12-30|Gödecke AG, 1000 Berlin|5-Phenyl-4-oxo-Delta 2, ALPHA-thiazolidine acetic acid ester|EP0269024A3|1986-11-21|1990-05-09|Godecke Aktiengesellschaft|Use of 3-methyl-4-oxothiazolidin-2-ylidene-acetamide derivatives in the treatment of aids|

---

AT144142T|1991-01-10|1996-11-15|Transcend Therapeutics Inc|USE OF THIAZOLIDIN-4-CARBOXYLATE DERIVATIVES FOR THE TREATMENT OF LUNG DISEASES|

---

US5747459A|1991-02-04|1998-05-05|Nestec, Ltd.|Method for insuring adequate intracellular glutathione in tissue|

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US6133299A|1993-02-25|2000-10-17|Warner-Lambert Company|Methods for treating neurodegenerative diseases and disorders using N--N'-pyridinyl ureas and other anticonvulsant compounds|

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US5447712A|1993-12-09|1995-09-05|Free Radical Sciences|Method of reducing cyclophosphamide induced hemorrhagic cystitis|

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KR100329808B1|2000-01-14|2002-03-25|박호군|An efficient method for preparing 2-phenacylidene-3,5-dialkylsubstituted-1,3-thiazolidine-4-ones|

法律状态:

优先权:

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申请号 | 申请日 | 专利标题

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DE3317000A|DE3317000C2|1983-05-10|1983-05-10|4-Oxo-thiazolidin-2-ylidene-acetamide derivatives, process for their preparation and their use in combating convulsions caused by the central nervous system|

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